Maryam Bibi Iqbal Ibrahim,1, Steven Ray Haakon Wilson,1,2 and Hanne Røberg-Larsen,1,2
1Section of Chemical Life
Science, Department of Chemistry, University of Oslo, Norway 2Hybrid Technology Hub, Faculty of Medicine,
University of Oslo, Norway
Email: mbibrahi@kjemi.uio.no
For drug discovery/testing, NAMs are alternative models to traditional model organisms, such as rodents and canines. Organoids are 3D-cell culture systems that allow the cells to imitate living tissue found in organs, an example of a NAMs that can be used as alternatives to model organisms. Zebrafish larvaes aged < 120 hours post fertilization are also considered as more ethical alternatives to traditional model organisms even though they are in a grey area between model organisms and NAMs. However, there is a need to measure actual drug content in NAMs, to quantify drug uptake for relating to biological effects. One drug class of interest is beta blockers, which are primarily used to treat patients with high blood pressure and cardiovascular diseases by inhibiting beta receptors that are stimulated by adrenaline and are found in the sympathetic nervous system. We will here present different approaches to measuring beta blockers, exemplified with propranolol, varying sample preparation and liquid chromatography-mass spectrometry (LC-MS) settings.