Siri Furre,a,b, Matthias Anagho-Mattanovich,a, Marharyta Vasnietsova a,Terje Vasskog,b, Thomas Moritz,a
a Novo Nordisk Foundation Center for Basic Metabolic Research, Moritz Group, Faculty of Health and Medical
Sciences, University of Copenhagen
b Natural Products and Medicinal Chemistry Research Group, Department of Pharmacy, Faculty of Health Sciences,
University of Tromsø – The Arctic University of Norway
Email: siri.furre@sund.ku.dk
Despite its immense potential, the routine application of metabolomics in clinical settings and large-scale research is hampered by several challenges. Two of the major hurdles are incomplete metabolite coverage and lengthy analytical run times. This project focus on shortening the analytical run time, specifically for amino acids. The analyses are based on 6-aminoquinolyl-N-hydroxysuccinimudyl carbamate (AQC) derivatisation of primary and secondary amines, a well-established method for amino acid analysis (e.g. Salazar et al. 2012). The project involves optimization of liquid chromatography (UHPLC) and mass spectrometry methods. For the ultra-fast analysis, a combined trapped ion mobility time-of-flight mass spectrometer (tims-TOF-MS) will be used (Schroeder et al. 2020). The ultra-fast method, approx. 2 minutes between injection, will be compared with our normal UHPLC combined with triple-quadrupole mass spectrometer (TQ-MS) method with a between injection time of 17 minutes. Advantages and limitations of the ultra-fast method based on combined UHPLC-tims-TOF will be discussed. Validation of the fast method will be done accordingly to guidelines from regulatory bodies, e.g. presision and accuracy.
References:
Salazar, C.; Armenta, J.M.; Shulaev, V. An UPLC-ESI-MS/MS Assay Using 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate Derivatization for Targeted Amino Acid Analysis: Application to Screening of Arabidopsis thaliana Mutants. Metabolites 2012, 2, 398-428. https://doi.org/10.3390/metabo2030398
Schroeder, M.; Meyer, S.W.; Heyman, H.M.; Barsch, A.; Sumner, L.W. Generation of a Collision Cross Section Library for Multi-Dimensional Plant Metabolomics Using UHPLC-Trapped Ion Mobility-MS/MS. Metabolites 2020, 10, 13. https://doi.org/10.3390/metabo10010013