P. Appelblad
Merck Life Science AS, Drammensveien 123, 0277 Oslo, Norway Email: patrik.appelblad@merckgroup.com
Hydrophilic Interaction Liquid Chromatography (HILIC) has evolved significantly over the past 30 years, leading to advancements in the separation of polar and hydrophilic analytes and today the technique is also considered for the analysis of larger molecules such as proteins and oligonucleotides1. This presentation will explore the classification of HILIC phases and the development of stationary phases, highlighting some key innovations that have shaped the field. There will be a discussion around the comparative performance of nine surface-grafted zwitterionic chromatography columns evaluated for HILIC mode glycan profiling of immunoglobulin G (IgG)-derived glycans2. The presentation will highlight findings on glycan retention, chromatographic peak attributes, and resolution across various stationary phases, including those featuring sulfobetaine (SB) and phosphorylcholine (PC) functionalities. Additionally, the impact of particle and pore size on throughput and analysis time will be discussed, showcasing how solid core particles can enhance efficiency in glycan profiling and quality control. The presentation aims to provide insights into the advancements in HILIC methodologies and their implications for analytical chemistry, using examples with relevance for biopharmaceutical applications.
References
1. An update on the progress in fundamental understanding of hydrophilic interaction liquid chromatography Yong Guo and David Muscatiello; J. Chromatography A, 1765 (2026) 466520.
2. Evaluating zwitterionic stationary phases for glycan profiling of IgGs from various sources by HPLC-ESI-MS/MS Fernando de Carlos Hernandez, Terkel Hansen, Hanne Haslene-Hox, Patrik Appelblad, and Anna Nordborg. J. Chromatography A, 1761 (2025) 466393.